Analysis of carbohydrates in plants by high-performance anion-exchange chromatography coupled with electrospray mass spectrometry.
Identifieur interne : 004110 ( Main/Exploration ); précédent : 004109; suivant : 004111Analysis of carbohydrates in plants by high-performance anion-exchange chromatography coupled with electrospray mass spectrometry.
Auteurs : Cédric Guignard [Luxembourg (pays)] ; Laurent Jouve ; Marie Béatrice Bogéat-Triboulot ; Erwin Dreyer ; Jean-François Hausman ; Lucien HoffmannSource :
- Journal of chromatography. A [ 0021-9673 ] ; 2005.
Descripteurs français
- KwdFr :
- Alcools benzyliques (analyse), Chromatographie d'échange d'ions (instrumentation), Chromatographie d'échange d'ions (méthodes), Chromatographie en phase liquide à haute performance (méthodes), Eau (pharmacologie), Feuilles de plante (composition chimique), Feuilles de plante (effets des médicaments et des substances chimiques), Glucides (analyse), Glucosides (MeSH), Hexose (analyse), Populus (composition chimique), Populus (effets des médicaments et des substances chimiques), Reproductibilité des résultats (MeSH), Résines échangeuses d'anions (MeSH), Spectrométrie de masse ESI (méthodes).
- MESH :
- analyse : Alcools benzyliques, Glucides, Hexose.
- composition chimique : Feuilles de plante, Populus.
- effets des médicaments et des substances chimiques : Feuilles de plante, Populus.
- méthodes : Chromatographie d'échange d'ions, Chromatographie en phase liquide à haute performance, Spectrométrie de masse ESI.
- pharmacologie : Chromatographie d'échange d'ions, Eau.
- Glucosides, Reproductibilité des résultats, Résines échangeuses d'anions.
English descriptors
- KwdEn :
- Anion Exchange Resins (MeSH), Benzyl Alcohols (analysis), Carbohydrates (analysis), Chromatography, High Pressure Liquid (methods), Chromatography, Ion Exchange (instrumentation), Chromatography, Ion Exchange (methods), Glucosides (MeSH), Hexoses (analysis), Plant Leaves (chemistry), Plant Leaves (drug effects), Populus (chemistry), Populus (drug effects), Reproducibility of Results (MeSH), Spectrometry, Mass, Electrospray Ionization (methods), Water (pharmacology).
- MESH :
- chemical , analysis : Benzyl Alcohols, Carbohydrates, Hexoses.
- chemical , pharmacology : Water.
- chemical : Anion Exchange Resins, Glucosides.
- chemistry : Plant Leaves, Populus.
- drug effects : Plant Leaves, Populus.
- instrumentation : Chromatography, Ion Exchange.
- methods : Chromatography, High Pressure Liquid, Chromatography, Ion Exchange, Spectrometry, Mass, Electrospray Ionization.
- Reproducibility of Results.
Abstract
A mass spectrometer was coupled to high-performance anion-exchange chromatography (HPAEC) with the help of electrochemical neutralization of the eluent and post-column addition of lithium chloride for carbohydrate analysis. Parallel selective channels (single ion monitoring) were used to decrease the detection limits and separate unresolved peaks. The mass specific detection allowed the simultaneous analysis of a wide range of sugar alcohols, mono-, di- and oligosaccharides. Carbohydrates extracted from leaves of poplar submitted to drought stress were analyzed using pulsed amperometric detection (PAD), then mass spectrometry. It allowed the confirmation of peak attribution and the identification of salicin as a major compound in the extracts. Different responses to water deficit and re-hydration were obtained for several carbohydrates, suggesting different roles in osmoprotection processes.
DOI: 10.1016/j.chroma.2005.05.068
PubMed: 16106860
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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A</title><idno type="ISSN">0021-9673</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anion Exchange Resins (MeSH)</term><term>Benzyl Alcohols (analysis)</term><term>Carbohydrates (analysis)</term><term>Chromatography, High Pressure Liquid (methods)</term><term>Chromatography, Ion Exchange (instrumentation)</term><term>Chromatography, Ion Exchange (methods)</term><term>Glucosides (MeSH)</term><term>Hexoses (analysis)</term><term>Plant Leaves (chemistry)</term><term>Plant Leaves (drug effects)</term><term>Populus (chemistry)</term><term>Populus (drug effects)</term><term>Reproducibility of Results (MeSH)</term><term>Spectrometry, Mass, Electrospray Ionization (methods)</term><term>Water (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alcools benzyliques (analyse)</term><term>Chromatographie d'échange d'ions (instrumentation)</term><term>Chromatographie d'échange d'ions (méthodes)</term><term>Chromatographie en phase liquide à haute performance (méthodes)</term><term>Eau (pharmacologie)</term><term>Feuilles de plante (composition chimique)</term><term>Feuilles de plante (effets des médicaments et des substances chimiques)</term><term>Glucides (analyse)</term><term>Glucosides (MeSH)</term><term>Hexose (analyse)</term><term>Populus (composition chimique)</term><term>Populus (effets des médicaments et des substances chimiques)</term><term>Reproductibilité des résultats (MeSH)</term><term>Résines échangeuses d'anions (MeSH)</term><term>Spectrométrie de masse ESI (méthodes)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Benzyl Alcohols</term><term>Carbohydrates</term><term>Hexoses</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Water</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Anion Exchange Resins</term><term>Glucosides</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Alcools benzyliques</term><term>Glucides</term><term>Hexose</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Feuilles de plante</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Feuilles de plante</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Chromatography, Ion Exchange</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>Chromatography, Ion Exchange</term><term>Spectrometry, Mass, Electrospray Ionization</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Chromatographie d'échange d'ions</term><term>Chromatographie en phase liquide à haute performance</term><term>Spectrométrie de masse ESI</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Chromatographie d'échange d'ions</term><term>Eau</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Reproducibility of Results</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Glucosides</term><term>Reproductibilité des résultats</term><term>Résines échangeuses d'anions</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A mass spectrometer was coupled to high-performance anion-exchange chromatography (HPAEC) with the help of electrochemical neutralization of the eluent and post-column addition of lithium chloride for carbohydrate analysis. Parallel selective channels (single ion monitoring) were used to decrease the detection limits and separate unresolved peaks. The mass specific detection allowed the simultaneous analysis of a wide range of sugar alcohols, mono-, di- and oligosaccharides. Carbohydrates extracted from leaves of poplar submitted to drought stress were analyzed using pulsed amperometric detection (PAD), then mass spectrometry. It allowed the confirmation of peak attribution and the identification of salicin as a major compound in the extracts. Different responses to water deficit and re-hydration were obtained for several carbohydrates, suggesting different roles in osmoprotection processes.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16106860</PMID><DateCompleted><Year>2005</Year><Month>09</Month><Day>12</Day></DateCompleted><DateRevised><Year>2019</Year><Month>07</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0021-9673</ISSN><JournalIssue CitedMedium="Print"><Volume>1085</Volume><Issue>1</Issue><PubDate><Year>2005</Year><Month>Aug</Month><Day>26</Day></PubDate></JournalIssue><Title>Journal of chromatography. A</Title><ISOAbbreviation>J Chromatogr A</ISOAbbreviation></Journal><ArticleTitle>Analysis of carbohydrates in plants by high-performance anion-exchange chromatography coupled with electrospray mass spectrometry.</ArticleTitle><Pagination><MedlinePgn>137-42</MedlinePgn></Pagination><Abstract><AbstractText>A mass spectrometer was coupled to high-performance anion-exchange chromatography (HPAEC) with the help of electrochemical neutralization of the eluent and post-column addition of lithium chloride for carbohydrate analysis. Parallel selective channels (single ion monitoring) were used to decrease the detection limits and separate unresolved peaks. The mass specific detection allowed the simultaneous analysis of a wide range of sugar alcohols, mono-, di- and oligosaccharides. Carbohydrates extracted from leaves of poplar submitted to drought stress were analyzed using pulsed amperometric detection (PAD), then mass spectrometry. It allowed the confirmation of peak attribution and the identification of salicin as a major compound in the extracts. Different responses to water deficit and re-hydration were obtained for several carbohydrates, suggesting different roles in osmoprotection processes.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Guignard</LastName><ForeName>Cédric</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Public Research Center--Gabriel Lippmann, Environment and Biotechnologies Research Unit, 41 rue du Brill, L-4422 Belvaux, Luxembourg. guignard@lippmann.lu</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jouve</LastName><ForeName>Laurent</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Bogéat-Triboulot</LastName><ForeName>Marie Béatrice</ForeName><Initials>MB</Initials></Author><Author ValidYN="Y"><LastName>Dreyer</LastName><ForeName>Erwin</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Hausman</LastName><ForeName>Jean-François</ForeName><Initials>JF</Initials></Author><Author ValidYN="Y"><LastName>Hoffmann</LastName><ForeName>Lucien</ForeName><Initials>L</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Chromatogr A</MedlineTA><NlmUniqueID>9318488</NlmUniqueID><ISSNLinking>0021-9673</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000837">Anion Exchange Resins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001592">Benzyl Alcohols</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D021241" MajorTopicYN="N">Spectrometry, Mass, Electrospray Ionization</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014867" MajorTopicYN="N">Water</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>8</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>9</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>8</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16106860</ArticleId><ArticleId IdType="pii">S0021-9673(05)01092-7</ArticleId><ArticleId IdType="doi">10.1016/j.chroma.2005.05.068</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Luxembourg (pays)</li></country></list><tree><noCountry><name sortKey="Bogeat Triboulot, Marie Beatrice" sort="Bogeat Triboulot, Marie Beatrice" uniqKey="Bogeat Triboulot M" first="Marie Béatrice" last="Bogéat-Triboulot">Marie Béatrice Bogéat-Triboulot</name><name sortKey="Dreyer, Erwin" sort="Dreyer, Erwin" uniqKey="Dreyer E" first="Erwin" last="Dreyer">Erwin Dreyer</name><name sortKey="Hausman, Jean Francois" sort="Hausman, Jean Francois" uniqKey="Hausman J" first="Jean-François" last="Hausman">Jean-François Hausman</name><name sortKey="Hoffmann, Lucien" sort="Hoffmann, Lucien" uniqKey="Hoffmann L" first="Lucien" last="Hoffmann">Lucien Hoffmann</name><name sortKey="Jouve, Laurent" sort="Jouve, Laurent" uniqKey="Jouve L" first="Laurent" last="Jouve">Laurent Jouve</name></noCountry><country name="Luxembourg (pays)"><noRegion><name sortKey="Guignard, Cedric" sort="Guignard, Cedric" uniqKey="Guignard C" first="Cédric" last="Guignard">Cédric Guignard</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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